Evaluation of Disinfectant Efficacy against Biofilm-Residing Wild-Type Salmonella from the Porcine Industry

Salmonella enterica is a causative pathogen of Salmonellosis, zoonosis causing global disease and financial losses every year. Pigs may be carriers contribute to the spread humans food products. persist as biofilms. Biofilms are bacterial aggregates embedded in self-produced matrix known withstand disinfectants. We studied effect glutaraldehyde peracetic acid, two active substances frequently used disinfectant formulations pig industry, on representative biofilm-residing wild-type collected from housings United Kingdom (UK). screened biofilm production strains using microtiter plate (MTP) assay Congo Red Coomassie Blue (CRCB) agar method. Previously published stainless-steel coupon (SSCA), polyvinylchloride (PCA), glass bead (GBA) assays were for disinfection studies. The mean reduction tested met criterion ≥4 log10 CFU at concentration 0.05% with SSCA GBA, 0.005% PCA along 0.5% all three mean. At these concentrations, both disinfectants suitable against Salmonella. When evaluating efficacy disinfectants, biofilms should included, higher concentrations necessary compared planktonic bacteria.